Species-specific and universal DNA probes and amplification prim

Details Species-specific and universal DNA probes and amplification prim Species-specific and universal DNA probes and amplification prim

1996-11-04

1999-11-30

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 221, C12Q 168, C12P 1934, C07H 2102

Patent

active

059940662

ABSTRACT:
The present invention relates to a method for universal detection of bacteria in biological samples and for specific detection of Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus saprophyticus, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in urine or any other biological samples, said method comprising denaturation of bacterial DNA to single stranded form and either fixing it on a support or leaving it in solution, contacting said single stranded genetic material with a labeled probe selected from the group consisting of i) fragments of chromosomal DNA of the above-mentioned bacteria and ii) synthetic oligonucleotides whose sequences are derived either from the said fragments of chromosomal DNAs or from sequences available in data banks, all (i and ii) probes being capable to hybridize specifically to their chromosomal DNA or, in case of universal probes, to any bacterial chromosomal DNA.

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