Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2011-03-29
2011-03-29
Woolwine, Samuel C (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
Reexamination Certificate
active
07915014
ABSTRACT:
Described herein are labeled probes and unlabeled oligonucleotides that are useful for use in nucleic acid amplification reactions. These probes and oligonucleotides are modified to alter their sensitivity to primer-independent 5′ exonuclease activity of a thermostable DNA polymerase relative to its corresponding unmodified counterpart. Non-symmetric polymerase chain reaction (PCR) amplification and detection methods employing these labeled probes and unlabeled oligonucleotides are also described. Kits for nucleic acid amplification reactions including labeled probes and unlabeled oligonucleotides are also described.
REFERENCES:
patent: 2004/0072182 (2004-04-01), Lyamichev et al.
patent: 2004/0106109 (2004-06-01), Belly et al.
patent: 2004/0219570 (2004-11-01), Salerno
patent: WO-01/94638 (2001-12-01), None
Pierce et al. Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres. Hum. Mol. Reprod. 6:1155-64 (2000).
Tyagi et al. Molecular Beacons:Probes that fluoresce upon hybridization. Nature Biotechnology 14:303-308 (1996).
Heid et al. Real Time Quantitative PCR. Genome Research 6:986-94 (1996).
Lyamichev et al. Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes. Nature Biotechnology 17:292-6 (1999).
New England Biolabs: Description of Taq Polymerase [online] [retrieved Jun. 9, 2010] retrieved from http://www.neb.com
ebecomm/productsM0273.asp.
K. E. Pierce et al., “Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR,” Molecular Human Reproduction, vol. 9, No. 12, 2003, pp. 815-820.
K. E. Pierce et al., “Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection,” Proc. Natl. Acad. Sci. USA, vol. 102, No. 34, Jun. 14, 2005, pp. 8609-8614.
J. A. Sanchez et al., “Linear-After-The-Exponential (LATE)-PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis,” Proc. Natl. Acad. Sci. USA, vol. 101, No. 7, Feb. 17, 2004, pp. 1933-1938.
International Search Report (7 pgs.).
Written Opinion (5 pgs.).
Kenneth E. Pierce, et al., “Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR”, Molecular Human Reproduction, Oxford University Press, GB-BE, vol. 9, No. 12, Dec. 1, 2003; pp. 815-820, XP002397152; ISSN: 1360-9947; Abstract, p. 816, column 1—column 2; figure 1.
J.A. Sanchez, et al., “Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis” Proceedings of the National Academy of Sciences of USA, National Academy of Science, Washington, D.C., US, vol. 101, No. 7, Feb. 17, 2004; pp. 1933-1938, XP002990854; ISSN: 0027-8424; Abstract.
Extended European Search Report Communication issued Jan. 4, 2010, in European Application No. 07796898.0-2403.
Communication pursuant to Article 94(3) EPC in EP Appln No: 07 796 898.0 dated Nov. 17, 2010.
Brandeis University
Foley & Lardner LLP
Woolwine Samuel C
LandOfFree
Specialized oligonucleotides and their use in nucleic acid... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Specialized oligonucleotides and their use in nucleic acid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Specialized oligonucleotides and their use in nucleic acid... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2674888