Soybean transformation by microparticle bombardment

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4351723, 4352404, 800205, 800DIG26, C12H 514

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active

053227832

ABSTRACT:
Plant cells in tissue culture are more efficiently transformed in a method which involves treatment with a cytokinin followed by incubation for a period sufficient to permit undifferentiated cells in cotyledonary node tissue to differentiate into meristematic cells and to permit the cells to enter the phases between the G1 and division phases of development.

REFERENCES:
patent: 4945050 (1990-07-01), Sanford et al.
McCabe et al (Aug. 1988) Bio/Technology 6:923-926.
Wang et al (1988) Plant Molecular Biology 11:433-439.
Meyer et al (1985) Mol. Gen Genet 201: 513-518.
Potrykus (Jun. 1990)) Bio/Technology 8: 535-538.

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