Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1997-03-28
1999-10-19
Robinson, D. W.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
435419, 435426, 435430, 800312, C12N 510, C12N 1582, A01H 500
Patent
active
059688300
ABSTRACT:
A method of transforming and regenerating soybean plants relies on selection of hypocotyl explants as the target material. Hypocotyl explants can be transformed either by microparticle bombardment with DNA-coated microparticles of inert metals, or by co-culturing with an Agrobacterium strain. The transformed explants can be successfully regenerated, using a protocol including culturing on a shoot induction medium, followed by transfer to a shoot elongation medium to form rooted plantlets, which are transplanted to soil.
REFERENCES:
patent: 5416011 (1995-05-01), Hinchee et al.
Liu et al. Somatic embryogenesis in soybean via somatic embryo cycling. In Vitro Cell. Dev. Biol. 28P:153-160, Jul. 1992.
Sharma et al. High frequency plant regeneration in tissue cultures of Glycine clandestina--a wild relative of soybean. Phytomorphology. 43(1&2):29-33, Jan.-Jul. 1993.
Liu et al. Somatic embryo cycling: evaluation of a novel transformation and assay system for seed-specific gene expression in soybean. Plant Cell, Tissue and Organ Culture. 47:33-42, Mar. 1996.
Yuqing et al. Rapid and efficient regeneration from cotyledonary explants of soybean cultivars (Glycine max L.). J. Genet. & Breed. 49:339-342, 1995.
Brown, D.C.W., and T.A. Thorpe. 1986. Plant regeneration by organogenesis, pp. 49-65. In: Vasil, I.K. (Ed.) Cell Culture and Somatic Cell Genetics, vol. 3, Academic Press, Inc., Orlando, FL.
Dan, Y., and N.A. Reichert. 1988. Organogenic regeneration of soybean from hypocotyl explants. In Vitro Cell. Dev. Biol. 34P:14-21.
Dodds, J.H., and L.W. Roberts. 1995. Somatic embryogenesis, pp. 101-113. In: Experiments in Plant Tissue Culture, 3rd Ed., Cambridge University Press, New York, NY.
Gray, D.J. 1996. Nonzygotic embryogenesis, pp. 133-147. In: Trigiano, R.N., and D.J. Gray (Eds.) Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, Inc., Boca Raton, FL.
Kameya, T., and J. Widholm. 1981. Plant regeneration from hypocotyl sections of Glycine species. Plant Sci. Lett. 21:289-294.
Liu, K.S. 1997. Agronomic characteristics, production, and marketing, pp. 1-24. In: Soybeans: Chemistry, Technology, and Utilization, Chapman and Hall, New York, NY.
Pierik, R.L.M. 1987. Vegetative propagation, pp. 183-230. In: In Vitro Culture of Higher Plants, Martinus Nijhoff Publishers, Dordrecht, The Netherlands.
Poehlman, J.M. 1987. Breeding Soybeans, pp. 421-450. In: Breeding Field Crops, Van Nostrand Reinhold, New York, NY.
Sengupta-Gopalan, C., N.A. Reichert, R.F. Barker , T.C. Hall, and J.D. Kemp. 1985. Developmentally regulated expression of the bean .beta.-phaseolin gene in tobacco seed. Proc. Natl. Acad. Sci. USA 82:3320-3324.
Schwarz, O.J., and R.M. Beaty. 1996. Propagation from nonmeristematic tissues--organogenesis, pp. 95-104. In: Trigiano, R.N., and D.J. Gray (Eds.) Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, Inc., Boca Raton, FL.
Widholm, J.M., S. Rick. 1983. Shoot regeneration from Glycine canescens tissue cultures. Plant Cell Rep. 2:19-20.
Dan Yinghui
Reichert Nancy A.
Mississippi State University
Robinson D. W.
Wai Thanda
LandOfFree
Soybean transformation and regeneration methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Soybean transformation and regeneration methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Soybean transformation and regeneration methods will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2054970