Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2007-07-31
2007-07-31
Saidha, Tekchand (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S041000, C435S183000, C435S193000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
11034678
ABSTRACT:
Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol:(1) action and substrate specificity: produces raffinose from sucrose and galactinol;(2) optimum pH: about 6 to 8;(3) optimum temperature: about 35 to 40° C.;(4) molecular weight:(i) about 75 kDa to 95 kDa estimated by gel filtration chromatography;(ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and(iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and(5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.
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Kida Takao
Nozaki Jinshi
Osumi Chieko
Ajinomoto Co. Inc.
Fronda Christian L
Saidha Tekchand
LandOfFree
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