Soraphen A resistant fungi and acetyl-CoA carboxylase

Details Soraphen A resistant fungi and acetyl-CoA carboxylase Soraphen A resistant fungi and acetyl-CoA carboxylase

1994-12-13

1997-06-24

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

536 232, C12N 900, C07H 2104

Patent

active

056416660

ABSTRACT:
The present invention discloses DNA molecules comprising a gene encoding yeast acetyl-coenzyme A carboxylase resistant to soraphen A inhibition. Mutations confering soraphen A resistance to acetyl-CoA carboxylase can be dominant or recessive, and they can be point mutations, deletion or insertion mutations. In addition methods of isolating a gene encoding fungal acetyl-coenzyme A carboxylase resistant to soraphen A inhibition are provided as well as methods for purifying fungal acetyl-coenzyme A carboxylase resistant to soraphen A inhibition. Purified enzyme can be used in assays to identify inhibitors of soraphen A resistant acetyl-coenzyme A carboxylase.

REFERENCES:
Al-Feel, W., et al., "Cloning of the Yeast FAS3 Gene and Primary Structure of Yeast Acetyl-CoA Carboxylase", PNAS, 89:4534-4538 (1992).
Klebe, R.J., et al., "A General Method for Polyethylene-glycol-induced Genetic Transformation of Bacteria and Yeast", Gene, 25:333-341 (1983).
Matsuhashi, M., "Acetyl-CoA Carboxylast from Yeast", Methods in Enzymology, Lowenstein, J.M., Ed., 14:3-8 (1969).
Sikorski, R.S., et al., "A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces cerevisiae", Genetics, 122:19-27 (1989).
Vahlensieck, H-F., Ph.D. Thesis, "A Genetic Approach to Determine the Biochemical Targert of the Antimicrobial Agent, Soraphen A", Philosophisch-Naturwissenschaftlichen Fakultat der Universitat Basel (1993).
Vahlensieck, H.F. et al. (1994) "Identification of the yeast ACC1 gene product (acetyl-CoA carboxylase) as the target of the polyketide fungicide soraphen A" Curr. Genet. 25:95-100.
Roessler, P.G. (1993) "Genetic engineering approaches for enhanced production of biodiesel fuel from microalgae" Abstr. Pap. Am. Chem. Soc. 205 Meet., Pt. 2:BTEC29.

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