Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1985-10-01
1990-07-10
Warren, Charles F.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
4351722, 43524047, 43524051, 43524054, 935 94, 935 98, 800DIG38, 800DIG39, A01H 104, C12N 1500, C12N 500
Patent
active
049408364
ABSTRACT:
Somatic hybrids of Rutaceae plants were obtained by allowing the protoplasts from embryogenic cells of a Rutaceae plant, which are of the nucellar origin, resistant to fusion treatment, and have a high proliferation activity, to fuse with the protoplasts of another Rutaceae plant having no proliferation activity, and then cultivating the fused protoplasts in a medium not containing a phytohormone but containing a saccharide in a concentration suitable for the selective embryogenesis of the hybrid cells.
REFERENCES:
Kobayashi et al. (1983) Japan Jour. Breed. 33:119-122.
Larkin et al. (1981) Theor. Appl. Genet. 60:197-214.
W. Handro (1981) in Ta Thorpe, ed., Plant Tissue Culture, Methods and Applications in Agriculture, Academic Press, NY, pp. 156-157.
Ohgawara et al. (1985) Theor. Appl. Genet, 71:1-4.
Gamborg et al. (1981) in TA Thorpe, ed., Plant Tissue Culture, Methods and Applications in Agriculture, Academic Press, NY, pp. 115-153.
Spiegel-Roy et al. (1980) Advances in Biochemical Engineering, vol. 16:29-48.
Button et al. (1977) in J. Reinert and YPS Bajai, eds. Plant Cell, Tissue, and Organ Culture, pp. 70-92.
"Ribosomal DNA Analysis of Somatic Hybrids," T. Ohgawara, Plant Tissue Culture Letters, vol. 1, pp. 43-46, 1984.
Ishii Shigetaka
Kobayashi Shozo
Ohgawara Toshifumi
Oiyama Iwao
Yoshinaga Katsuichi
Cohen Charles E.
Director General of Fruit Tree Research Station Ministry of Agri
Kikkoman Corporation
Warren Charles F.
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