Solvent system for peroxidase inhibitor reagent for fecal occult

Details Solvent system for peroxidase inhibitor reagent for fecal occult Solvent system for peroxidase inhibitor reagent for fecal occult

1982-12-20

1985-01-15

Hazel, Blondel

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

436 66, C12Q 128, G01N 3372

Patent

active

044938926

ABSTRACT:
A process and reagent for determining the presence of fecal occult blood in a test sample using a guaiac-peroxidase test matrix and a means for inhibiting peroxidase enzyme interferences which combines the partial denaturing of peroxidase enzyme protein using a protein-hydrogen bond cleaving agent, with the removal of metals necessary for efficient peroxidase enzyme activity wherein the improvement comprises dissolving said reagent for cleaving protein-hydrogen bonds in an alcohol-water solvent before adding said reagent to the sample.

REFERENCES:
patent: 3996006 (1976-12-01), Pagano
patent: 4017261 (1977-04-01), Svoboda et al.
patent: 4175923 (1979-11-01), Friend
patent: 4277250 (1981-07-01), Melnick et al.
patent: 4333734 (1982-06-01), Fleisher
Dixon et al., Enzymes, Academic Press Inc., N.Y., p. 148, 1964.

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