Solvent-resistant microorganisms

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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Details

4351723, 435190, 4352523, 4353201, 536 231, 536 232, 536 237, C12N 1570, C12N 1553, C12N 904, C07H 2104

Patent

active

058077359

ABSTRACT:
The present invention includes microorganisms that are resistant to non-aqueous solvents. In addition, the organisms of interest are able to grow and/or carry out various organic (e.g. hydrocarbon) transformations in non-aqueous/aqueous mixtures.
The invention also includes a gene which encodes an enzyme, hydroperoxide reductase, which renders the host microorganism resistant to many organic solvents. The invention also includes the operon which includes the mutant gene and the ahpF gene which encodes an NAD(P)H dehydrogenase. The invention also includes a plasmid vehicle and a host microorganism containing these genes.
The invention also includes cloning the genes encoding for solvent-resistance into other organisms rendering them solvent-resistant. In addition, the genes encoding for a specific organic or hydrocarbon transformation are placed into the solvent resistant microorganism.

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