Solvent-induced autolysis of cells

Details Solvent-induced autolysis of cells Solvent-induced autolysis of cells

1985-06-10

1989-01-03

Warren, Charles F.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Lysis of micro-organism

435189, 435938, 435255, C12N 106, C12N 902

Patent

active

047957093

ABSTRACT:
A process to recover proteins, such as enzymes, from yeast cells, which comprises forming an admixture of yeast cells, water, and a minor effective amount of a polychloro aliphatic hydrocarbon, at a suitable pH, and incubating for a suitable time and temperature, such as at room temperature, of about 16 to 90 hours. The resulting supernatant is separated as an aqueous liquid containing a high enzyme activity. Enzymes can be recovered, if desired. Typical applications include Kluyveromyces fragilis for lactase, Pichia pastoris for alcohol oxidase. Typical solvents include methylene dichloride, 1,1,1-trichloroethane, and chloroform.

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