Solution phase nucleic acid sandwich assays having reduced backg

Details Solution phase nucleic acid sandwich assays having reduced backg Solution phase nucleic acid sandwich assays having reduced backg

1995-04-26

1997-06-03

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435808, 435810, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168

Patent

active

056353529

ABSTRACT:
New techniques are provided for substantially reducing background signals encountered in solution phase hybridization assays. The techniques are premised on eliminating or significantly reducing the phenomena of nonspecific hybridization and nonspecific binding, so as to provide a detectable signal which is produced only in the presence of the target polynucleotide of interest. In certain embodiments, methods are provided for increasing the signal which can otherwise be diminished in noise reduction. Kits for carrying out the novel assays are provided as well.

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Distefano et al., "Ligand-Promoted Dimerization of Oligonucleotides Binding Cooperatively to DNA", J. Am. Chem. Soc. 114:11006-11007 (1992).

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