Solution phase nucleic acid sandwich assay

Details Solution phase nucleic acid sandwich assay Solution phase nucleic acid sandwich assay

1985-12-11

1989-09-19

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 536 27, 935 77, 935 78, C12Q 168

Patent

active

048681059

ABSTRACT:
Methods and compositions are provided for rapid detection of nucleic acid sequences. The method employs two reagent sets. The first set is a labelling set comprising: (1) a first nucleic acid sequence probe having an analyte complementary region and a first recognition sequence region and (2) a labelled sequence complementary to the first recognition sequence region. The second set is a capturing set comprising: (1) a second nucleic acid sequence probe having an analyte complementary region and a second recognition sequence region, (2) a specific binding pair member conjugated to a sequence complementary to the second recognition sequence, and (3) a separating means to which is bound a complementary specific binding pair member. The sample and probes are combined under annealing conditions, followed by addition of the other reagents, separation of the bound label from the supernatant and detection of the label in either phase.

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