Solution hybridization of nucleic acids with antisense probes ha

Details Solution hybridization of nucleic acids with antisense probes ha Solution hybridization of nucleic acids with antisense probes ha

1998-05-22

2000-04-04

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, C12Q 168

Patent

active

060460040

ABSTRACT:
The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a fluid medium using laser induced fluorescence of antisense probes. The probes can have anionic backbones of reduced negative charge. Suitable probes can include methylphosphonate backbones. When the hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the number of mismatches between the probe and target. When the hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the fluid test medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence and proportional to the number of mismatches between the probe and target. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.

REFERENCES:
patent: 4220450 (1980-09-01), Maggio
patent: 4469863 (1984-09-01), Ts'o et al.
patent: 4787963 (1988-11-01), MacConnell
patent: 4963477 (1990-10-01), Tehen
patent: 5100775 (1992-03-01), Smyczek et al.
patent: 5142047 (1992-08-01), Summerton et al.
patent: 5166315 (1992-11-01), Summerton et al.
patent: 5166330 (1992-11-01), Engles et al.
patent: 5217592 (1993-06-01), Jones
patent: 5217866 (1993-06-01), Summerton et al.
patent: 5223618 (1993-06-01), Cook et al.
patent: 5310650 (1994-05-01), McMahon et al.
patent: 5332659 (1994-07-01), Kidwell
patent: 5405938 (1995-04-01), Summerton et al.
patent: 5470974 (1995-11-01), Summerton et al.
patent: 5501949 (1996-03-01), Marshall
patent: 5503980 (1996-04-01), Cantor
patent: 5521063 (1996-05-01), Summerton et al.
patent: 5538848 (1996-07-01), Livak et al.
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5541307 (1996-07-01), Cook et al.
patent: 5587469 (1996-12-01), Cook et al.
patent: 5594138 (1997-01-01), Dykstra et al.
patent: 5602240 (1997-02-01), De Mesmaeker et al.
patent: 5610289 (1997-03-01), Cook et al.
patent: 5618704 (1997-04-01), Sanghvi et al.
patent: 5623065 (1997-04-01), Cook et al.
patent: 5632957 (1997-05-01), Heller et al.
patent: 5674698 (1997-10-01), Zarling et al.
patent: 5677437 (1997-10-01), Teng et al.
patent: 5747247 (1998-05-01), Kowalczykowski et al.
patent: 5846729 (1998-12-01), Wu et al.
Perry-O'Keefe et al., "Peptide Nucleic Acid Pre-Gel Hybridization: An Alternative to Southern Hybridization," 93 Proc. Natl. Acad. Sci. USA 14670 (Dec. 1996).
Smulevitch et al., "Enhancement of Strand Invasion by Oligonucleotides Through Manipulation of Backbone Charge," 14 Nature Biotechnology 1700 (Dec. 1996) (disclosed in Landsdorp, "Close Encounters of the PNA Kind," 14 Nature Biotechnology 1653 (Dec. 1996)).
Lansdorp, "Close Encounters of the PNA Kind," 14 Nature Biotechnology 1653 (Dec. 1996).
Egholm et al., "PNA Hybridizes to Complementary Oligonucleotides Obeying the Watson-Crick Hydrogen-Bonding Rules," 365 Nature 566 (1993).
Tomac et al., "Ionic Effects on the Stability and Conformation of Peptide Nucleic Acid Complexes," 118 J.Am.Chem.Soc. 5544 (1996).
Coghlan, "One-Step DNA test in a tube," New Scientist, p. 21 (Nov. 5, 1994).
"PNA Oligomers as Hybridization Probes," vol. 1, Issue 2 of PerSeptive Biosystems Magazine, 1995.
Heppell-Parton, "Gene Mapping by Fluorescence in Situ Hybridization," p. 350-54, in Molecular Biology and Biotechnology: A Comprehensive Desk Reference (Myers, ed. 1995).
Matthews et al., "Analytical Strategies for the Use of DNA Probes," 169 Analystical Biochemistry 1 (1988).
Carlsson C. et al., "Screening for Genetic Mutations," Nature, 380:207, Mar., 1996.
Jensen et al., "Kinetics for Hybridization of Peptide Nucleic Acids (PNA) with DNA and RNA Studied with the BIAcore Technique," 36(16) Biochem. 5072 (Apr. 1997).
Grunstein et al., "Colony Hybridization: A Method for the Isolation of Cloned DNAs that Contain a Specific Gene," Proc. Nat'l Acad. Sci., 72(10):3961-3965, 1975.
Meinkoth et al., "Hybridization of Nucleic Acids Immobilized on Solid Supports," Analytical Biochemistry, 138:267, 1984.
Thomas, "Hybridization of Denatured RNA and Small DNA Fragments Transferred to Nitrocellulose," Proc. Nat'l Acad. Sci., 77(9):5201-5205, 1980.
Wetmur, Biopolymers, 14:2517-2524, 1975.
Chang et al., Biopolymers, 13:1847-1858, 1974.
Zhang et al., "Single-base mutational analysis of cancer and genetic diseases using membrane bound modified oligonucleotides," 19(14) Nucleic Acids Research 3929-3933 (Jul. 25, 1991).
R. Hogrefe et al., Nucleic Acids Research, 1993, vol. 21:2031-2039.

Affiliated with

Also associated with

Rating

Solution hybridization of nucleic acids with antisense probes ha does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Solution hybridization of nucleic acids with antisense probes ha, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Solution hybridization of nucleic acids with antisense probes ha will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-363921