Solution assay method using polarimetry

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

Reexamination Certificate

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C435S004000

Reexamination Certificate

active

06939683

ABSTRACT:
A method for the measurement of the concentration of a material such as dextran or raffinose in a solution, notably a sugar solution, includes the steps of measurement of the optical rotation of a solution sample, treatment of the sample with a reactive agent, measurement of the optical rotation of the sample after treatment to ascertain the difference that said treatment has made, and calculation of the concentration of the material by reference of said difference to a suitable standard. The reactive agent reacts with the material sufficiently to alter the optical rotation of the sample.

REFERENCES:
patent: 0 534 166 (1992-08-01), None
patent: 0 534 166 (1992-08-01), None
patent: WO 00/68695 (2000-11-01), None
Oikawa S. Determination of Sucrose, Invert Sugar and Raffinose in Sugar Solutions. Seito Gijutsu Kenkyu Kaishi 1962, vol. 11, pp. 28-37.
SU-B-734558 (Sugar Research Institute) (abstract only).
A New Polarimetric Dextran And Sucrose Test, by Christopher Bucke, Max Adlard, Victoria Singleton, and Jennifer Horn; Bharatiya Sugar Journal, Mar. 2001, pp. 127-134 (correction on named authors was corrected in later edition of the Journal).
A New Polarimetric Method For The Analysis Of Dextran And Sucrose, by Victoria Singleton, Jennifer Horn, Christopher Bucke, and Max Adlard; International Sugar Journal, vol. 103, No. 1230, Jun. 2001; pp. 251-254.
A New Polarimetric Method For The Analysis Of Dextran And Sucrose, by Victoria Singleton, Jennifer Horn, Christopher Bucke and Max Adlard; Proc. S.T.A.I. 63, pp. 110-119, 2001.
Double-Polarization Methods, Cane Sugar Handbook, 12th Edition, ed. Chen & Chou, J. Wilsy, 1993, pp. 831-842.

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