Soluble T cell receptors

Details Soluble T cell receptors Soluble T cell receptors

1995-02-17

2000-06-27

Scheiner, Laurie

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 691, 435 698, 435 701, 4352523, C07K 1400

Patent

active

060808403

ABSTRACT:
An intact, assembled T cell receptor (TcR) in soluble form is provided. The soluble TcR is prepared by splicing the extracellular domains of a T cell receptor to the glycosyl phosphatidylinositol (GPI) membrane anchor sequences of Thy-1. The molecule is expressed in the absence of CD3 on the cell surface, and can be cleaved from the membrane by treatment with phosphatidylinositol specific phospholipase C (PI-PLC). The .alpha. and .beta. chains of the soluble molecule are paired in the native conformation as judged by reactivity with the anti-V.sub..beta. 8 monoclonal antibody F23.1, and with the anti-clonotypic monoclonal antibody 1B2. The soluble TcR is a disulfide linked dimer with a molecular mass of 95 kDa on SDS-PAGE under nonreducing conditions, and 47 kDa after reduction.

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