Soluble steroidal peptides for nucleic acid delivery

Details Soluble steroidal peptides for nucleic acid delivery Soluble steroidal peptides for nucleic acid delivery

2008-01-22

2008-01-22

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Vector, per se

C536S023500, C536S024100

Reexamination Certificate

active

11099999

ABSTRACT:
Amphiphilic lipopeptide compositions for gene delivery are disclosed. An illustrative amphiphilic lipopeptide composition includes a human protamine 2 peptide conjugated to a hydrophobic moiety. Illustrative hydrophobic moieties include sterols, bile acids, and fatty acids. The amphiphilic lipopeptide composition is mixed with a nucleic acid such that the nucleic acid binds to the peptide portion of the lipopeptide. This mixture is placed in contact with mammalian cells to effect transfection of the cells with the nucleic acid. A method of making such amphiphilic lipopeptides is also described.

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Cotten, Langle0Rouault, Kirlappos, Wagner, Mechtler, Zenke, Beug, and Birnstiel, Transferrin-polycation-mediated introduction of DNA into human leukemic cells: Stimulation by agents.that affect the survival of transfected DNA or modulate transferrin receptor levels, Proc. Natl. Acad. Sci, USA, Jun. 1990, pp. 4033-4037, vol. 87.
Han, Mahto, and Kim, Water-Soluble Lipopolymer for Gene Delivery, Bioconjugate Chem., 2001, 12, pp. 337-345.

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