Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1980-10-02
1987-03-24
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
435 16, 435 17, 435 21, 435 26, C12N 996, C12Q 152, C12Q 150, C12Q 142, C12Q 132
Patent
active
046525246
ABSTRACT:
A method is disclosed for preparing a soluble stable enzyme. The method comprises the steps of reacting in a liquid media an enzyme with a polymer having pendant groups capable of covalently bonding with pendant groups on the enzyme. The enzyme and polymer are mixed with at least one composition which affects the activity of the enzyme. Such a composition can be selected from the enzyme substrate, product of the enzyme substrate reaction, an activator for the enzyme and/or inhibitor for the enzyme. In addition, a composition can be added which can also competitively react with the available pendant groups of the polymer.
REFERENCES:
patent: 3860484 (1975-01-01), O'Malley
patent: 4017364 (1977-04-01), Leemputten
patent: 4226938 (1980-10-01), Yoshida et al.
patent: 4250254 (1981-02-01), Modrovich
Gaussiran Wanda A.
Modrovich Ivan E.
Wegfahrt, Jr. Paul F.
Kepplinger Esther M.
Modrovich Ivan E.
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