Soluble neuropilin/growth factor receptor fusion proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S359000, C530S399000

Reexamination Certificate

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10303786

ABSTRACT:
Complexes of a protein selected from the group consisting of VEGF-B167, VEGF-C, VEGF-D and processed VEGF-B186and analogs thereof and the neuropilin-1 (NP-1) receptor, the extracellular domain or a ligand-binding fragment or analogue thereof; the use of such complexes in assays for growth factor proteins having substantially the same binding affinity for a cell surface receptor as VEGF-B167, VEGF-C, VEGF-D or processed VEGF-B186and/or in promoting or antagonizing a cellular response mediated by VEGF-B167, VEGF-C, VEGF-D and/or processed VEGF-B186; and specific binding partners, e.g. antibodies, for such complexes.

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Migdal et al., “Neuropilin-1 Is a Placenta Growth Factor-2 Receptor”, The Journal of Biological Chemistry, 273:22272-22278 (1998).
Neuropilin-1 Is Expressed by Endothelial and Tumor Cells as an Isoform Specific Receptor for Vascular Endothelial Growth Factor, by Shay Soker, et al., Cell vol. 92, 735-745, Mar. 20, 1998.
Tanja Veikkola, et al., “VEGF's Receptors and Angiogenesis”, Seminars in Cancer Biology, Jun. 1999, pp. 211-220, vol. 9, No. 1998.0091, Academic Press, XP-002198307.
Marc G. Achen, et al., “Vascular Endothelial Growth Factor D (VEGF-D) is a Ligand for the Tyrosine Kinases VEGF Receptor 2 (FIk1) and VEGF Receptor 3 (FIt4)”, Proc. Natl. Acad. Sci., Jan. 1998, pp. 548-553, vol. 95, XP002905751.
Taija Makinen, et al., “Differential Binding of Vascular Endothelial Growth Factor B Splice and Proteolytic Isoforms to Neuropilin-1”, The Journal of Biological Chemistry, Jul. 23, 1999, pp. 21217-21222, vol. 274, No. 30, The American Society for Biochemistry and Molecular Biology, Inc., XP002944607.

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