Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1993-01-14
1997-04-22
Ulm, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
435 691, C07K 14735
Patent
active
056230532
ABSTRACT:
The present invention pertains to a new composition of matter in the form of a soluble Fc receptor (pHsFcR) is created. The pHsFcR has a pH-determinable binding capability for at least one antibody or complex thereof. The pHsFcR is produced using the cDNA for a transmembrane, pH-determinable Fc receptor found in nature which has the capability of binding to at least one antibody or complex thereof. The most preferred embodiment of the present invention is a secreted form of pHsFcR which is produced using a modified cDNA of the transmembrane Fc receptor; the modified cDNA is obtained by the creation of a new in-frame stop codon and the deletion of the DNA sequence encoding the transmembrane (and typically the cytoplasmic) domain of the transmembrane Fc receptor. This creates a new gene that encodes a completely soluble protein consisting of the extracellular domains of the transmembrane Fc receptor. The new gene is used in formation of an expression vector which is introduced into a procaryotic or eucaryotic cell, followed by selection for a procaryotic or eucaryotic cell comprising the modified cDNA of the desired secreted pHsFcR. The selected cells are grown, during which pHsFcR is secreted into the growth medium. The secreted pHsFcR can then be separated from the growth medium using an affinity column comprising an antibody to which the pHsFcR can bind.
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Bjorkman Pamela J.
Gastinel Louis N.
California Institute of Technology
Church Shirley L
Spector Lorraine
Ulm John
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