Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-09-13
1985-07-23
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 436536, 436538, 436539, 436540, 436815, 436824, 436828, G01N 3354, C12N 996
Patent
active
045309000
ABSTRACT:
The invention pertains to an improved heterogeneous enzymeimmunoassay involving the use of a reversibly soluble polymeric substance acting as the support for the antibody. In the direct method the antigen to be detected and an enzyme labeled antigen are bound by antibody which is chemically linked to the soluble polymeric substance. The polymer is rendered insoluble and removed from the test solution. After resolubilization into a solution containing substrate for the enzyme label, the assay for antigen is completed by determination of enzymatic activity. In the indirect method, the antigen to be detected and an enzyme labeled antigen are incubated with a primary antibody unattached to the polymeric substance. After addition of a second antibody which is chemically linked to the polymeric substance, the polymer is rendered insoluble and the assay is performed as in the direct method described above.
REFERENCES:
patent: 3791932 (1977-02-01), Schuurs et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 4003792 (1977-01-01), Mill et al.
patent: 4134792 (1979-01-01), Boquslaski et al.
patent: 4233401 (1980-11-01), Yoshida et al.
patent: 4238565 (1980-12-01), Hornby et al.
patent: 4289747 (1981-09-01), Chu
patent: 4298685 (1981-11-01), Parikh et al.
patent: 4352883 (1982-10-01), Lim
patent: 4371515 (1983-02-01), Chu
patent: 4407957 (1983-10-01), Lim
M. Charles, R. W. Coughlin and F. X. Hasselberger, Biotechnology and Bioengineering, vol. XVI, pp. 1153-1156, (1974).
A. L. Margolin et al., Biotechnology and Bioengineering, vol. XXIV, pp. 237-240, (1982).
D. Worl et al., Clin. Chem., 27(5), 673-677, (1981).
That. T. Ngo et al., J. Immunological Methods, 42, 93-103, (1981).
A. J. O'Brierne and H. R. Cooper, J. Hist. Cytochemistry, 27(8), 1148-1162, (1979).
Cook Paul J.
Kepplinger Esther M.
Nesbitt Stephen L.
Seragen Diagnostics Inc.
LandOfFree
Soluble insoluble polymers in enzymeimmunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Soluble insoluble polymers in enzymeimmunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Soluble insoluble polymers in enzymeimmunoassay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-634583