Soluble immunoassay reagent comprising lectin covalently bonded

Details Soluble immunoassay reagent comprising lectin covalently bonded Soluble immunoassay reagent comprising lectin covalently bonded

1981-08-14

1985-01-15

Fagelson, Anna P.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

2601125R, 424 11, 424 85, 424 88, 424177, 424195, 436500, 436501, 436503, 436528, 436529, 436827, 435 7, C07G 700, G01N 3348, G01N 3354, G01N 3368, G01N 3378, A61K 3929, A61K 39106, A61K 3578

Patent

active

044937938

ABSTRACT:
A lectin is covalently bonded to an immunological conjugate such as an antibody-antigen or its equivalent. Then, the lectin-conjugate is isolated from the reaction product mixture by one of a number of alternative techniques involving one or more of the following types of reaction; (1) reversible reaction of the lectin with an insolubilized sugar to isolate lectin from the remainder of the mixture, (2) reaction of one immunological component (e.g., antibody) bonded to the lectin with an insolubilized corresponding component (e.g., antigen) to separate the antibody components from the remainder of the reaction mixture, and (3) filtration of the reaction components to separate on the basis of product molecular weight, size and/or shape of the components.

REFERENCES:
patent: 3994870 (1976-11-01), Neurath
patent: 4041146 (1977-08-01), Glaever
patent: 4289747 (1981-09-01), Chu
Miles Biochemicals/Miles Res. Products, Miles Labs, 1976, pp. 106-108.
Nicolson, Nature, N.B. vol. 239, Oct. 18, 1972, pp. 193-197.
Lis et al., Ann Rev. Biochem., vol. 42, 1973, pp. 541-551, 558-574.
Miles Res Products, Quality Line Bulletin, Summer, 1978, Miles Labs, Elkhart, Ind., pp. 3-4.
Aspberg et al., Acta Chem. Scand., vol. 24, 1970, pp. 1839-1840.
Avigad, Analytical Biochem., vol. 86, 1978, pp. 443-449.
Gonatas, Chem. Abs., vol. 80, 1974, Ab. No. 12154t.
Smith, Chem. Abs., vol. 74, 1971, Ab. No. 40239u.

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