Solubilization of proteins for pharmaceutical compositions using

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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530395, 530389, 530390, 530391, 530402, 530403, 530405, 514 2, 514 8, 514 56, 424 85, 435811, C07K 1500, A61K 4502, A61K 3702, A61K 3900

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047451807

ABSTRACT:
A pharmaceutical composition is prepared wherein a biologically active conjugated protein which is .beta.-interferon, interleukin-2, or an immunotoxin is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated protein, which is poorly or not at all water-soluble at pH 6-8 without such solubilizing agent, is selectively conjugated to at least one heparin fragment having a terminal 2,5-anhydro-D-mannose residue which has an aldehyde not involved in intramolecular hemiacetal formation.

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Ceustermans et al., J. Biol. Chem., 257:3401 (1982).
Bjork et al., FEBS Letters, 143(a), 96 (1982).

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