Solubilization of protein with ethanol-acetonitril-water solvent

Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal

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23230B, 252364, 356 36, C07G 700, G01N 3316

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active

039753433

ABSTRACT:
A solvent system composed of ethanol-acetonitrile-water dissolved certain membrane proteins of biological origin. The solvent system was optically transparent to ultraviolet radiation from 200 to 400 nanometers (nm). This transparency permitted ascertainment of spectral characteristics of membrane proteins at these wavelengths.

REFERENCES:
patent: 3856471 (1974-12-01), Winitz et al.
Kaplan, D. M. and Woodward, D. O., Evaluation of . . . Mitochodrial Membrane Protein Preparations; Analytical Biochemistry 52, 102-110 (1973).
West et al., Textbook of Biochemistry, 4th ed., 1966, p. 262.
Scheflan et al., The Handbook of Solvents, 1953, pp. 87 and 88.
Visser et al., An Automated Procedure for Circular Dichroism and Optical Rotary Dispersion Spectroscopy; Analytical Biochemistry 60, 59-77 (1974).

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