Solubilization of protein after bacterial expression using sarko

Details Solubilization of protein after bacterial expression using sarko Solubilization of protein after bacterial expression using sarko

1991-01-22

1993-08-31

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 711, 435232, 435259, 530412, 530825, C12P 2100, C12N 988

Patent

active

052408345

ABSTRACT:
This invention relates to two methods for solubilizing proteins which are rendered insoluble by bacterial expression. One method comprises directly lysing the host bacterial cells with the detergent Sarkosyl. The other method comprises conventional lysing of the bacteria followed by an extraction process using Sarkosyl and fractionation. These methods render the proteins soluble. They do not entail harsh denaturation of the proteins, and therefore do not require renaturation of the proteins in many cases. Rather, they render the proteins soluble, in their native form.

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