Solubilization and purification of the active gastrin releasing

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

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530413, 530420, 530424, 530350, 424 858, A61K 3702, A61K 39395

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active

052159639

ABSTRACT:
A procedure for the preparation of the solubilized and purified gastrin releasing peptide receptor, in an active form, from a gastrin releasing peptide receptor source such as Swiss 3T3 fibroblasts.

REFERENCES:
T. W. Moody, D. N. Carney, F. Cuttitta, K. Quattrocchi and J. D. Minna, "High Affinity Receptors for Bombesin/GRP-like Peptides on Human Small Cell Lung Cancer", Life Sciences 37(2): 105-113 (1985).
F. Cuttitta, D. N. Carney, J. Mulshine, T. W. Moody, J. Fedorko, A. Fischler and J. D. Minna, "Bombesin-like Peptides Can Function as Autocrine Growth Factors in Human Small-Cell Lung Cancer", Nature 316: 823-826 (Aug. 29, 1985).
J. Zachary, P. J. Woll and E. Rozengurt, "A Role for Neuropeptides in the Control of Cell Proliferation", Developmental Biology 124: 295-308 (1987).
R. M. Kris, R. Hagan, J. Villines, T. W. Moody and J. Schlessinger, "Identification of Bombesin Receptor on Murine and Human Cells by Cross-linking Experiment", The Journal of Biological Chemistry. 262(23): 11215-11220 (Aug. 15, 1987).
I. Zachary and E. Rozengurt, "Identification of a Receptor for Peptides of the Bombesin Family in Swiss 3T3 Cells by Affinity Cross-linking", The Journal of Biological Chemistry 262(9): 3947-3950 (Mar. 25, 1987).
J. Mazella, J. Chabry, P. Kitabgi and J. P. Vincent, "Solubilization and Characterization of Active Neurotensin Receptors from Mouse Brains", The Journal of Biological Chemistry 263(1): 144-149 (Jan. 5, 1988).
J. D. Minna, F. Cuttitta, J. F. Battey, J. L. Mulshine, I. Linnoila, A. F. Gazdar, J. Trepel and E. A. Sausville, "Gastrin-Releasing Peptide and Other Antocrine Growth Factors in Lung Cancer: Pathogenetic and Treatment Implications", Important Advances in Oncology, J. B. Lippincott Co., Philadelphia (1988).

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