Solubilization and purification of a target protein fused to...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C435S069100, C435S267000, C536S023100

Reexamination Certificate

active

07825218

ABSTRACT:
Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.

REFERENCES:
patent: 2006023635 (2006-03-01), None
Hennig, et al., Protein Expression and Purification, 14:367-370 (1998).
Kapust & Waugh, Protein Sci. 8:1668-74 (1999).

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