Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-02-14
1997-09-16
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 911, 435 912, 435 71, 436810, 536 231, 536 243, 536 2432, 536 2433, 536 253, 536 552, 424482, 424489, 564100, 564110, C12Q 168, C12Q 170, C12P 1934, C07H 2104
Patent
active
056679769
ABSTRACT:
Compositions and methods for covalently immobilizing an oligonucleotide onto a polymer-coated solid support or similar structure are provided. Specifically, the polymer-coated support, such as a bead, possesses a large number of activatable moieties, preferably primary and secondary amines. An oligonucleotide is activated with a monofunctional or multifunctional reagent, preferably the homotrifunctional reagent cyanuric chloride. The resultant covalently immobilized oligonucleotides on the support serve as nucleic acid probes, and hybridization assays can be conducted wherein specific target nucleic acids are detected in complex biological samples. The beads or similar structures can be employed free in solution, such as in a microtiter well format; in a flow-through format, such as in a column; or in a dipstick. Additionally, dichlorotriazine oligonucleotides and processes for activating oligonucleotides by treatment with cyanuric chloride and derivatives are included in the present invention.
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Aerschot et al. "Silica gel functionalized with different spacers as solid supports for oligonucleotide synthesis". Nucleosides and Neucleotides, 7(1):75-90 1988.
Aershot et al. Nucleosides and Nucleotides 7:75-90.
Ness Jeffrey Van
Petrie Charles R.
Reed Michael W.
Tabone John C.
Vermeulen Nicolaas M.J.
Becton Dickinson and Company
Highet, Esq. David W.
Rees Dianne
Zitomer Stephanie W.
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