Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Reexamination Certificate
2008-05-06
2008-05-06
Le, Long V. (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
C436S532000, C436S530000, C436S546000, C436S805000, C436S823000, C564S281000
Reexamination Certificate
active
07368296
ABSTRACT:
Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.
REFERENCES:
patent: 2780604 (1957-02-01), Clarke et al.
patent: 3065272 (1962-11-01), Garner et al.
patent: 3178396 (1965-04-01), Lloyd et al.
patent: 3236820 (1966-02-01), Lloyd et al.
patent: 3770439 (1973-11-01), Taylor
patent: 3898088 (1975-08-01), Cohen et al.
patent: 3958995 (1976-05-01), Campbell et al.
patent: 4308335 (1981-12-01), Yamamoto et al.
patent: 4322489 (1982-03-01), Land et al.
patent: 4340522 (1982-07-01), Bronstein-Bonte et al.
patent: 4424326 (1984-01-01), Land et al.
patent: 4503138 (1985-03-01), Bronstein-Bonte et al.
patent: 4563411 (1986-01-01), Bronstein-Bonte
patent: 4737560 (1988-04-01), Heilmann et al.
patent: 4871824 (1989-10-01), Heilmann et al.
patent: 4981933 (1991-01-01), Fazio et al.
patent: 5200471 (1993-04-01), Coleman et al.
patent: 5336596 (1994-08-01), Bronstein et al.
patent: 5336742 (1994-08-01), Heilmann et al.
patent: 5547836 (1996-08-01), Bronstein et al.
patent: 5593828 (1997-01-01), Bronstein et al.
patent: 5672514 (1997-09-01), Tsuchiya et al.
patent: 5705357 (1998-01-01), Kissel et al.
patent: 5827650 (1998-10-01), Bronstein et al.
patent: 5849495 (1998-12-01), Bronstein et al.
patent: 5981185 (1999-11-01), Matson et al.
patent: 6162610 (2000-12-01), Bronstein et al.
patent: 2001/0012537 (2001-08-01), Anderson et al.
patent: 2447611 (1975-04-01), None
patent: 0 294 186 (1988-12-01), None
patent: 0 567 187 (1993-10-01), None
patent: 1 120 422 (2001-08-01), None
patent: WO 92/13018 (1992-08-01), None
patent: WO 96/25667 (1996-08-01), None
patent: WO 99/53319 (1999-10-01), None
Shizuka, “Excited-State Proton-Transfer Reactions and Proton-Induced Quenching of Aromatic Compounds”, Accounts of Chemical Research, 18, 141-147 (1985).
Jones, et al., “p-Vinylbenzyltrialkyl Ammonium Salts in Vinyl Polymerization”, Journal of Polymer Science, 25, 201-215 (1957). (Cited as “1958” on p. 19 of the specification).
Dahlberg, et al., “Electrostatic Catalysis of Proton-Transfer Reactions: Hydrogen Exchange in Chloroform and Ionization of 2-Nitropropane”, J. Am. Chem. Soc, 105, 5387-5390 (1983).
Rosenthal, et al., “Acid-Base Equilibria in Concentrated Salt Solutions. IV. Some Bases of Various Charge Types in Dilute Acid Solutions”, The Journal of Physical Chemistry, 69, 5, 1588-1595 (1965).
Bardsley, et al., “Synthesis and Oxidation of Aminolkyl-onium Compounds by Pig Kidney Diamine Oxidase”, Biochem, J., 122, 557-567 (1971).
Ing, et al., “CCCXII. —A Modification of the Gabriel Synthesis of Amines”, J. Chem. Soc., 2348-2351 (1926).
Sasaki, et al., “Convenient Synthesis of Some Purine 8,5′-Imino Cyclonucleosides”, J. Org. Chem., 43, 12, 2320-2324 (1978).
Khan, Kinetic Evidence for the Occurence of a Stepwise Mechanism in Hydrazinolysis of Phthalimide, J. Org. Chem., 60, 4536-4541 (1995).
Motawia, et al., “A Convenient Route to 3′-Amino-3′-Deoxythymidine”, Synthesis, 384-387 (1989).
Durette, et al., “Bacterial Cell Wall Constituents. II. Synthesis ofO-(N-Acetyl-β-Muramyl-L-Alanyl-D-Isoglutamine)—(1-4) -N-Acetyl-D- Glucosamine”, Tetrahedron Letters, 42, 4013-4016 (1979).
Edwards Brooks
Geiser Timothy G.
Menchen Steven M.
Sparks Alison L.
Voyta John C.
Applied Biosystems
Haq Shafiqul
Le Long V.
Merchant & Gould P.C.
Raimund Christopher W.
LandOfFree
Solid phases optimized for chemiluminescent detection does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Solid phases optimized for chemiluminescent detection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Solid phases optimized for chemiluminescent detection will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2811236