Solid phase transverse diffusion assay

Details Solid phase transverse diffusion assay Solid phase transverse diffusion assay

1995-05-22

1999-09-28

Saunders, David

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

422 56, 422 58, 422 60, 435 4, 435 5, 435 6, 435 71, 435 74, 435 75, 4352872, 4352878, 435805, 435970, 436514, 436518, 436524, 436525, 436530, 436807, 436828, G01N 33543, G01N 33558, G01N 33566

Patent

active

059587903

ABSTRACT:
The present invention relates to a method and kit for carrying out a qualitative or semi-quantitative assay. The invention uses a multi-layer device including an an uppermost cover layer of a water-impermeable material having a hole therein with a diameter of approximately 1-5 mm, an intermediate insoluble porous support layer having a first substance bound thereon in a reaction zone, the hole exposing at least a part of the reaction zone, and a layer of a hydrophilic material in contact with and positioned on the side of the insoluble porous support layer opposite the side with the cover layer. The device permits transverse, but not substantial radial, diffusion of liquid through the reaction zone. Approximately 1-50 .mu.l of a test sample and a second substance are applied to the reaction zone through the hole. A colloidal gold label is attached to the second substance. The presence or approximate quantity of the immobilized second substance is assessed by the presence, for a qualitative determination, or intensity for a semi-quantitative determination, of a color signal generated by the immobilized colloidal gold label in the part of the reaction zone exposed by the hole.

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