Solid phase system for ligand assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435805, 435 7, 436514, 436518, 436815, 436822, 436810, G01N 33535, G01N 33543, G01N 33558, C12Q 170

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active

047741740

ABSTRACT:
A method for conducting a ligand assay in an inert porous medium wherein a binding material is immunologically immobilized within the medium, which includes the steps of immunologically immobilizing a binding material within a finite zone of the medium, applying an analyte to the zone containing the immobilized binding material, applying a labeled indicator to the zone which becomes immobilized within the zone in an amount which can be correlated to the amount of analyte in the zone, applying a solvent to substantially the center of the zone to chromatographically separate the unbound labeled indicator from the zone, and measuring the amount of labeled indicator remaining in the zone.

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patent: 4363874 (1982-12-01), Greenquist
patent: 4366241 (1982-12-01), Tom et al.
patent: 4517288 (1985-05-01), Giegel et al.
Giegel et al., Clin. Chem., 28(9): 1894-1898, "Radial Partition Immunoassay", (1982).

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