Solid phase system for ligand assay

Details Solid phase system for ligand assay Solid phase system for ligand assay

1985-03-07

1988-11-22

Kepplinger, Esther M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

436501, 436514, 436518, 436815, 436817, 436824, G01N 33566, G01N 33543

Patent

active

047866063

ABSTRACT:
A method for conducting a ligand assay in an inert porous medium wherein a binding material is immunologically immobilized within the medium, which includes the steps of immunologically immobilizing a binding material within a finite zone of the medium, applying an analyte to the zone containing the immobilized binding material, applying a labeled indicator to the zone which becomes immobilized within the zone in an amount which can be correlated to the amount of analyte in the zone, applying a solvent to substantially the center of the zone to chromatographically separate the unbound labeled indicator from the zone, and measuring the amount of labeled indicator remaining in the zone.

REFERENCES:
patent: 3552925 (1971-01-01), Fetter
patent: 3645687 (1972-02-01), Nerenberg
patent: 4059405 (1977-11-01), Sodickson
patent: 4071315 (1978-01-01), Chateau
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4299916 (1981-11-01), Litman et al.
patent: 4363874 (1982-12-01), Greenquist
patent: 4366241 (1982-12-01), Tom et al.
patent: 4517288 (1985-05-01), Giegel et al.
Giegel et al., Clin. Chem., 28(9):1894-1898, "Radial Partition Immunoassay", (1982).

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