Synthetic resins or natural rubbers -- part of the class 520 ser – Synthetic resins – Mixing of two or more solid polymers; mixing of solid...
Patent
1981-04-30
1983-08-30
Michl, Paul R.
Synthetic resins or natural rubbers -- part of the class 520 ser
Synthetic resins
Mixing of two or more solid polymers; mixing of solid...
525375, 5253333, 5253336, 536 23, 536 24, 536 28, 536 29, C08F 840
Patent
active
044017960
ABSTRACT:
A resin support formed from a suitable resin such as polystyrene or a silica gel may be provided by forming an amino resin and by reacting the amino resin with an activated ester of a nucleoside to obtain the dimethoxytrityl resin. To add each successive nucleotide to the resin support, the nucleoside is phosphorylated to provide a monotriazolide. The phosphorylation may be provided by the reaction of a phosphoditriazolide with a 3'-hydroxyl neucleoside. The dimethoxytrityl resin is then hydroxylated to substitute the hydroxyl group for the dimethoxytrityl group in the resin support. The monotriazolide is then reacted with the hydroxylated resin support to provide the dimethoxytrityl resin. This reaction may occur in the presence of a nucleophilic catalyst, such as a catalyst selected from a group consisting of dimethylaminopyridine, N'-methylimidazole and tetrazole. A hydroxyl group may be then substituted again for the dimethoxytrityl group in the dimethoxytrityl resin to provide for the addition of other nucleotides to the resin support. The procedure may be repeated to provide polynucleotides with extended chains of nucleotides.
REFERENCES:
patent: 3985690 (1976-10-01), Dean
patent: 4037037 (1977-07-01), Patchornik et al.
patent: 4043948 (1977-08-01), Rayskys et al.
patent: 4066827 (1978-01-01), Seita
patent: 4217421 (1980-08-01), Beasley
patent: 4276395 (1981-06-01), Vollhardt et al.
City of Hope Research Institute
Michl Paul R.
Roston Ellsworth R.
Schwartz Charles H.
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