Solid phase reactant for inhibition and removal of serine protea

Details Solid phase reactant for inhibition and removal of serine protea Solid phase reactant for inhibition and removal of serine protea

1987-06-15

1991-06-04

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435217, 435815, 2523153, C12N 950, C12N 968, B07J 1300

Patent

active

050213466

ABSTRACT:
An affinity gel for the isolation of serine proteases from a biological sample. The affinity gel is modified for specificity for serine proteases by coupling an organophosphorous compound to the gel. The reaction is carried out in two steps. The hydrophilic gel is reacted with a phosphoryl trifluoride and then the coupled gel is further reacted with an alcohol to form the organophosphorous compound.

REFERENCES:
Nakayama, T. et al. (1977), Anal. Biochem. 78, 165-170.
Gordon, S. G. et al. (1981), J. Clin. Invest. 67, 1665-1671.
Affinity Chromatography, Principles and Methods (1979), Pharmacia Fine Chemicals, pp. 33-34.
Gel Filtration, Theory and Practice (1982), Pharmacia Fine Chemicals, pp. 16-17.
Affinity Chromatography, Principals and Methods, (1979), Pharmacia Fine Chemicals, pp. 19, 27-29.
Stryer, L., Biochemistry, pp. 129-130 (1975).
Lehninger, A., Principles of Biochemistry, pp. 221-223 (1982).
Pharmacia Fine Chemicals, "Affinity Chromatography, Principles and Methods", at pp. 6-7 (Jun. 1979).
Sigma Chemical Company, "Sigma Chemica Company Catalogue", p. 411 (1990).
Millqvist et al., "An Affinity Gel for the Inhibition, Binding and Isolation of Serine Proteases", Anal. Biochem. 170, pp. 289-292 (1988).

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