Solid-phase precipitation assay device

Details Solid-phase precipitation assay device Solid-phase precipitation assay device

1990-07-16

1993-05-25

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

436 13, 436 71, 422 55, 422 58, 422 56, C12Q 160, G01N 3392, G01N 2100

Patent

active

052139654

ABSTRACT:
An assay device for measuring the concentration of a soluble analyte, such as HDL-associated cholesterol, in a fluid sample containing interfering compounds, such as LDL-or VLDL-associated cholesterol, which can be selectively precipitated. The device includes a sieving matrix capable of separating soluble and precipitated material migrating through the matrix, and a reservoir which holds a precipitating agent which is effective, within a given concentration range, to selectively precipitate the interfering compounds. The reservoir is designed to delay the release of agent, on contact with the fluid sample, to maintain the concentration of precipitating agent in contact with the fluid sample within the given concentration range.

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patent: 4987085 (1991-01-01), Allen
Bachorik, P. S., Methods in Enzymology (1986), vol. 129 "Precipitation Methods for Quantification of Lipoproteins" pp. 78-100, Academic Press, Inc.

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