Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Implant or insert
Patent
1996-08-20
1998-12-29
Naff, David M.
Drug, bio-affecting and body treating compositions
Preparations characterized by special physical form
Implant or insert
435174, 435177, 435180, 435181, 435395, 530402, 530810, 530812, 530815, 530816, A61F 206, C12N 500, C12N 1106, C07K 1706
Patent
active
058537449
ABSTRACT:
A method for making a medical device having a biomolecule immobilized on a substrate surface is provided. The method includes: providing an immobilized biomolecule comprising a biomolecule covalently attached to a support material; attaching a photoreactive crosslinking agent to the immobilized biomolecule to form a photoreactive analog of the biomolecule; and removing the photoreactive analog of the biomolecule from the support material. The photoreactive analog of the biomolecule can then be attached to a substrate surface, such as a biomaterial that forms part of a medical device. The immobilized biomolecule may contain a peptide having an N.sup..alpha. -terminus. The photoreactive crosslinking agent is attached to the peptide at the N.sup..alpha. -terminus to form the photoreactive analog of the biomolecule. The peptide can be an adhesion peptide containing the sequence Trp-Gln-Pro-Pro-Arg-Ala-Arg-Ile. Attachment of the peptide to a substrate surface promotes cell adhesion to the surface. The photoreactive crosslinking agent can be heterobifunctional or contain two photoreactive groups. The photoreactive analog of the biomolecule is attached to the substrate surface by activating a photoreactive group of the analog such as by exposing the analog to UV radiation.
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Fields Gregg B.
Mooradian Daniel L.
Naff David M.
Regents of the University of Minnesota
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