Solid phase indicator red blood cells and method

Details Solid phase indicator red blood cells and method Solid phase indicator red blood cells and method

1986-09-08

1989-03-28

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is a biological cell or cell fragment

435 7, 436501, 436512, 436513, 436521, 436529, 436532, 436538, 436824, G01N 33555, G01N 33549

Patent

active

048164135

ABSTRACT:
A method and composition for use in preparing red blood cells for use as indicator cells to detect the presence of antigens and antibodies in biological fluids is the subject of the present invention. Freshly collected human or animal erythrocytes are washed six times with isotonic saline, and suspended to a hematocrit of four percent (volume/volume) with isotonic saline. An incomplete antibody, specific for the antigen or antibody to be detected, in a quantity less than that which would cause irreversible agglutination, is added to the erythrocyte solution. The mixture is incubated, washed, and suspended to a hematocrit of 3-5 (v/v) with isotonic saline. A second antibody, immuno specific for the first antibody and in an amount sufficiently great to immunologically bind substantially all of the antibody carrying red blood cells but not so great as to cause irreversible agglutination, is attached to the erythrocytes. This mixture is incubated, washed, and suspended to a hematocrit of 0.1-1% (v/v) in a media containing red blood cell nutrients. The resulting indicator red blood cells are used with solid phase immunological testing using various types of solid substrates.

REFERENCES:
patent: 4326027 (1982-04-01), Wenz
patent: 4403037 (1983-09-01), Coates
patent: 4587222 (1986-06-01), Gaffroy
patent: 4608246 (1986-08-01), Bayer et al.

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