Solid phase immunofluorescent assay method

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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23230B, 424 7, 424 11, 424 12, 424 13, 424 78, G01N 2100, G01N 2138, G01N 3122, G01N 3316

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042017637

ABSTRACT:
An assay method suitable for antigens (or haptens) including proteins and polypeptides utilizing antibodies for the unknown protein or polypeptide in which the antibodies are covalently bound to water insoluble hydrophylic polymeric particles. Appropriate fluorescently labeled immune reactants are introduced during the method and together with the unknown antigen are immunologically bound, directly or indirectly, to the particles for separation from the reaction liquid. The particles have a size of about 0.1-10 microns and can be resuspended for direct measurement of the fluorescent labels in a fluorometer from which the unknown can be determined.

REFERENCES:
patent: 3641235 (1972-02-01), Weiss
patent: 3720760 (1973-03-01), Bennick
patent: 3853987 (1974-12-01), Dreyer
patent: 3901654 (1975-08-01), Gross
patent: 3925018 (1975-12-01), Saunders
patent: 3998943 (1976-12-01), Ullman
Molday, Nature, vol. 249, May 3, 1974, pp. 81-83.
Capel; J. Immuno Methods, vol. 5, 1974, pp. 165-178.

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