Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1996-10-30
1998-08-04
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436523, 436528, 4352883, 4352884, 435962, G01N 3353
Patent
active
057892610
ABSTRACT:
Solid supports for solid phase immunoassays comprise polyethyleneimine-coated negatively charged polymeric materials. The combination of negatively-charged polymer support and polyethyleneimine coating serves to eliminate nonspecific adsorption of biological molecules such as high molecular weight kininogen, which have affinity for solid surfaces and which interfere with immunoassays. The negatively charged polymeric support to which the polyethyleneimine coating is applied may advantageously comprise, for example, a carboxylate-modified styrene microparticle. Antigens or antibodies are coupled by covalent coupling agents to the polyethyleneimine coating to form solid phase immunoreagents. Immunoassays for biological molecules such as high and low molecular weight kininogen are provided.
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Kaufmann et al., "Structural Dissection of the Multidomain Kininogens", The Journal of Biological Chemistry vol. 268, No. 12, pp. 9079-9091 (Apr. 25, 1993).
Bruil et al., "Poly(ethyleneimine) modified filters for the removal of leukocytes from blood", Journal of Biomedical Materials Research 27:1253-1268 (1993).
Scheiner Toni R.
Temple University Of The Commonwealth System Of Higher Education
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