Solid phase Fmoc chemistry process to prepare peptides

Details Solid phase Fmoc chemistry process to prepare peptides Solid phase Fmoc chemistry process to prepare peptides

2011-03-01

2011-03-01

Gupta, Anish (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Synthesis of peptides

C530S333000, C530S334000, C530S335000, C530S329000

Reexamination Certificate

active

07897724

ABSTRACT:
The present invention relates to an improved process for the preparation of N6-(aminoiminomethyl)-N2-(3-mercapto-1-oxopropyl)-L-lysylglycyl-L-α-aspartyl-L-tryptophyl-L-prolyl-L-cysteinamide, cyclic(1→6)-disulfide of formula (1), which involves assembling a peptide chain comprising of six amino acids and a thioalkyl carboxylic acid in a required sequence on a solid support to obtain a peptide bound resin of formula (2), capping the free amino groups after each coupling, cleaving Dde group in the peptide of formula (2) from the solid support to obtain peptide-solid support of formula (3), guanylating the peptide of formula (3) at ε-lysine-NH2in an organic solvent to obtain peptide-solid support of formula (4), cleaving and deprotecting all groups in the peptide of formula (4) from the solid support to obtain peptide-amide formula (5), oxidizing the SH-peptide of formula (5) with an appropriate oxidizing agent to obtain the crude peptide-amide of formula (1) and purifying the crude peptide-amide of formula (1) by chromatographic technique. The solid support is either resin or a cellulose support like cotton, gauze, fabric, paper and perloza beads. The described process is simple, easy, environment friendly, takes lesser time and more cost effective.

REFERENCES:
patent: 2004/0249121 (2004-12-01), Tovi et al.
patent: WO 03/093302 (2003-11-01), None

Affiliated with

Also associated with

Rating

Solid phase Fmoc chemistry process to prepare peptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Solid phase Fmoc chemistry process to prepare peptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Solid phase Fmoc chemistry process to prepare peptides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2680465