Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1998-06-16
1999-06-22
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 23, 435 24, 435 14, 435 15, 435 25, 435 4, 435808, 4352831, 435968, 422 50, C12Q 144, C12Q 137, C12Q 154, C12Q 100
Patent
active
059142456
ABSTRACT:
A MicroColonyImager instrument and solid phase methods to screen cells expressing mutagenized enzymes for enhanced activity. The MicroColonyImager instrument and methods permit high throughput screening of enzyme libraries by time course analyses of single-pixels, using either absorption, fluorescence or FRET. This high throughput assay can detect small differences in enzyme rates within microcolonies grown at a nearly confluent density on an assay disk. Each microcolony is analyzed simultaneously at single-pixel resolution, requiring less than 100 ml substrate/measurement. By simultaneously assaying different substrates tagged with spectrally distinct chromogenic or fluorogenic reporters, the substrate specificity of an enzyme can be changed.
REFERENCES:
Caldwell et al; "J. Microbiological Methods"; vol. 15(4); pp. 249-261; (1992) (Abstract).
Weaver et al; Methods (San Diego); vol. 2(3); pp. 234-247; (1991) (Abstract).
Bylina Edward J.
Coleman William J.
Dilworth Michael R.
Silva Christopher M.
Yang Mary M.
Kairos Scientific Inc.
Leary Louise N.
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