Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1993-06-08
1995-08-15
Woodward, Michael P.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
436177, 436178, 436170, 436541, 436826, 435962, G01N 33553, G01N 33537, G01N 2177
Patent
active
054418968
ABSTRACT:
The present invention is directed to a new assay procedure and improved solid phase assay devices for use with a so-called physical developer. The invention provides a new assay procedure for detecting the presence of an analyte in a fluid sample using a tracer having a metal label which signal is enhanced by applying a physical developer. The assay is performed with a device having a support for a test area and having attached thereto a binder specific for the analyte to be detected. The assay comprises the steps of directing the fluid sample and the tracer through a porous medium into contact with the test area at a controlled flow rate to allow binding of the analyte with the binder and the tracer to the analyte, directing a physical developer through said porous medium to amplify the signal generated by the tracer, and separating the device to allow the test area to be read. The invention further relates to devices and immunoassay test kits for carrying out the improved immunoassay procedure. A first device FIG. 2 according to the present invention has a support 4 having upper and lower surfaces and a test area on its upper surface. A removable filter 3 overlies the upper surface of the support. A second device FIG. 1 according to the present invention has a support 4 having upper and lower surfaces and a test area on its lower surface which is in contact with the absorptive layer 2.
REFERENCES:
patent: 4493815 (1985-01-01), Fernwood et al.
patent: 4704255 (1987-11-01), Jolley
patent: 4775636 (1988-10-01), Moeremans
Scopsi, L., "Silver-Enhanced Colloidal Gold Method", Hayat, Mass. Colloidal Gold, vol. 1, (Academic Press, NY) Chapter 9, 251-295 (1989).
Traina et al., Focus 5:5-6, 9 (1983).
Brada et al., Anal Biochem 142:79-83 (1984).
Danscher, G. "Autometallography", Histochemistry 81, 331-335 (1984).
Lackie, et al., "Investigation by Immunogold-Silver Staining by Electron Microscopy", Histochemistry 83, 545-550 (1985).
Garrevoet Theo C.
Noppe Marcus J. M.
LandOfFree
Solid phase assay for use with a physical developer does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Solid phase assay for use with a physical developer, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Solid phase assay for use with a physical developer will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2181312