Solid phase assay for use with a physical developer

Details Solid phase assay for use with a physical developer Solid phase assay for use with a physical developer

1995-06-06

1998-01-20

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

422 55, 422 56, 422 57, 422 58, 422 61, 422101, 4352871, 4352872, 4352877, 4352878, 4352879, 435805, 435810, 435975, 436514, 436518, 436524, 436169, 436170, 436805, 436808, 436810, G01N 33543, G01N 33551, G01N 33553

Patent

active

057100497

ABSTRACT:
The present invention is directed to a new assay procedure and improved solid phase assay devices for use with a so-called physical developer. The invention provides a new assay procedure for detecting the presence of an analyte in a fluid sample using a tracer having a metal label which signal is enhanced by applying a physical developer. The assay is performed with a device having a support for a test area and having attached thereto a binder specific for the analyte to be detected. The assay comprises the steps of directing the fluid sample and the tracer through a porous medium into contact with the test area at a controlled flow rate to allow binding of the analyte with the binder and the tracer to the analyte, directing a physical developer through said porous medium to amplify the signal generated by the tracer, and separating the device to allow the test area to be read. The invention further relates to devices and immunoassay test kits for carrying out the improved immunoassay procedure. A first device FIG. 2 according to the present invention has a support 4 having upper and lower surfaces and a test area on its upper surface. A removable filter 3 overlies the upper surface of the support. A second device FIG. 1 according to the present invention has a support 4 having upper and lower surfaces and a test area on its lower surface which is in contact with the absorptive layer 2.

REFERENCES:
patent: 4493815 (1985-01-01), Fernwood et al.
patent: 4704255 (1987-11-01), Jolley
patent: 4775636 (1988-10-01), Moeremans et al.
patent: 4916056 (1990-04-01), Brown, III et al.
Scopsi "Silver-Enhanced Colloidal Gold Method" Hayat MA Colloidal Gold vol. 1 (Academic Press NY) Chapter 9, pp.251-295 (1989).
Danscher, G., "Autometallography", Histochemistry 81:331-335 (1984).
Lackie, et al., "Investigation of immunogold-silver staining byy electron microscopy", Histochemistry 83:545-500 (1985).
Traina et al., Focus 5:5-6, (1983).
Brada et al., Anal Biochem 142:79-83 (1984).

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