Solid phase assay for urea

Details Solid phase assay for urea Solid phase assay for urea

1993-12-10

1994-12-06

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

422 56, 422 57, 435 791, 435805, 435970, 436904, C12Q 158, G01N 2100

Patent

active

053709942

ABSTRACT:
A method for detecting urea in a liquid sample in which urease is adsorbed on a solid support such as a membrane and contacted with a solution suspected of containing urea, a pH-dependent reducing agent and a tetrazolium salt. When urea is present in the solution, the adsorbed urease converts it to ammonia, thus raising the pH and causing the pH-dependent reducing agent to reduce the tetrazolium salt to an insoluble colored formazan. The formazan precipitates as a detectable spot on the solid support, indicating the presence of urea in the liquid sample.

REFERENCES:
patent: 4590157 (1986-05-01), Chandler
patent: 4727025 (1988-02-01), Siedel
patent: 4892817 (1990-01-01), Pawlak
patent: 4946776 (1990-08-01), Ritterband
patent: 5139934 (1992-08-01), Stewart

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