Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Reexamination Certificate
2006-08-15
2006-08-15
Nguyen, Bao-Thuy L. (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
C435S007100, C435S007920, C435S007930, C435S007950, C435S962000, C435S967000, C436S514000, C436S523000, C436S538000
Reexamination Certificate
active
07091050
ABSTRACT:
The present invention provides an improved system for detecting the presence or level of an analyte in a sample. In “competition-like” assays of the present invention, a sample including an analyte is mixed with a second ligand to which the analyte binds, and the mixture is exposed to a solid phase containing a first ligand that can compete with the analyte for binding to the second ligand. According to the present invention, the time of exposure of the mixture to the solid phase is limited so that substantially no dissociation of analyte/second ligand complex occurs. The competition-like assays of the present invention are preferably performed with a solid phase containing a substantial excess of first ligand. In “sandwich-type” assays of the present invention, a sample including an analyte is contacted with a solid phase including a first ligand that binds the analyte and, simultaneously or subsequently, is contacted with a second ligand that binds the analyte (or the analyte/first ligand complex). The time of contact between the second ligand and the solid phase is limited so that substantially no non-specific binding between the second ligand and the solid phase occurs.
REFERENCES:
patent: 1716321 (1929-06-01), Pearson
patent: 2798718 (1957-07-01), Gross
patent: 3002092 (1961-09-01), Cary
patent: 3025142 (1962-03-01), Williams
patent: 3492396 (1970-01-01), Dalton et al.
patent: 3600063 (1971-08-01), Bowen
patent: 3740552 (1973-06-01), Pressman
patent: 4059685 (1977-11-01), Johnson
patent: 4115535 (1978-09-01), Giaever
patent: 4153675 (1979-05-01), Klelnerman
patent: 4173392 (1979-11-01), Ekinaka et al.
patent: 4200690 (1980-04-01), Root et al.
patent: 4202665 (1980-05-01), Wenz et al.
patent: 4246339 (1981-01-01), Cole et al.
patent: 4268171 (1981-05-01), Sternberg
patent: 4308026 (1981-12-01), Mochida et al.
patent: 4407943 (1983-10-01), Cole et al.
patent: 4424279 (1984-01-01), Bohn et al.
patent: 4425438 (1984-01-01), Bauman et al.
patent: 4434236 (1984-02-01), Freytag
patent: 4447546 (1984-05-01), Hirschfeld
patent: 4454234 (1984-06-01), Czerlinski
patent: 4459361 (1984-07-01), Gefter
patent: 4469787 (1984-09-01), Woods et al.
patent: 4505260 (1985-03-01), Metzger
patent: 4582809 (1986-04-01), Block et al.
patent: 4585623 (1986-04-01), Chandler
patent: 4652533 (1987-03-01), Jolley
patent: 4678268 (1987-07-01), Russo et al.
patent: 4713347 (1987-12-01), Mitchell et al.
patent: 4714345 (1987-12-01), Schrader
patent: 4720465 (1988-01-01), Jensen et al.
patent: 4721681 (1988-01-01), Lentrichia et al.
patent: 4775515 (1988-10-01), Cottingham
patent: 4780423 (1988-10-01), Bluestein et al.
patent: 4912051 (1990-03-01), Zaromb
patent: 4960692 (1990-10-01), Lentrichia et al.
patent: 4963498 (1990-10-01), Hillman et al.
patent: 5120643 (1992-06-01), Ching et al.
patent: 5183740 (1993-02-01), Ligler et al.
patent: 5372783 (1994-12-01), Lackie
patent: 5554340 (1996-09-01), Lackie
patent: 6664114 (2003-12-01), Lackie et al.
patent: 0206077 (1986-06-01), None
patent: 0317286 (1988-11-01), None
patent: 0404258 (1989-06-01), None
patent: 2 190 490 (1987-11-01), None
patent: 61-20241 (1986-09-01), None
patent: WO 80/02747 (1980-12-01), None
patent: WO 89/06799 (1989-07-01), None
patent: WO 90/07380 (1990-07-01), None
patent: WO 93/14403 (1993-07-01), None
patent: WO 94/03104 (1994-02-01), None
Pollema et al., ananl. Chem. 64:1356-1361, 1992.
Friguet et al., Journal of Immuno. Meth., 77:305-319, 1985.
Freytag, Clin. Chem. 30(9):1494-1498, 1984.
PCT Search Report, May 30, 1995.
Dill, et al., “Antibody-Antigen Binding Constancts Determined in Solution-Phase with the Threshold Membrane-Capture System: Binding Constant for Anti-Fluorescein, Anti-Saxitoxin, and Anti-Ricin Antibodies”,Anal. Biochem.217: 128, 1994.
Ekins, et al., “Multianalyte Microspot Immunoassay-Microanalytical “Compact Disk” of the Future”,Clin. Chem., 37(11): 1955-1967, 1991.
Forrest, et al., “Liposome Enhanced Flow Injection Immunoanalysis”, Biotechnology, 14:1-11, 1988.
Freytag, et al., “Affinity-Column-Mediated Immunoenzymometric Assays: Influence of Affinity-Column Ligand and Valency of Antibody-Enzyme Conjugates”,Clin. Chem.30(9): 1494-1498, 1984.
Freytag, et al., “A Highly Sensitive Affinity-Column-Mediated Immunometric Assay, as Exemplified by Digoxin”,Clin. Chem.30(3): 417-420, 1984.
Friguet et al., “Measurements of the True Affinity Constant in Solution of Antigen-Antibody Complexes by Enzyme-Linked Immunosorbent Assay”,Jour. of Immun. Methods, 77: 305-319, 1985.
Glaser, et al., “Antigen-Antibody Binding and Mass Transport by Convection and Diffusion to a Surface: A Two-Dimensional Computer Model of Binding and Dissociation Kinetics”,Anal. Biochem, 213:152-161, 1993.
Glass, et al., “Effect of Numerical Aperture on Signal Level in Cylindrical Waveguide Evanescent Fluorosensors”,Applied Optics, 26(1): 2181-2187, 1987.
Goldberg, et al., “Methods for Measurment of Antibody/Antigen Based on ELISA and RIA”,Curr. Op. Immunol.3: 278, 1993.
Gübitz, et al., “Flow-Injection Immunassays”,Analytica Chimica Acta, 283: 421-428, 1993.
Gunaratna, et al., “Noncompetitive Flow Injection Immunoassay for a Hapten, α-(Difluoromethyl)ornithine.”Anal. Chem., 65: 1152-1157, 1993.
Hernando, et al., “An On-Line Immunoassay Method for Theophylline Using a Protein A Immunoreactor”,Journal of Pharmaceutical&Biomedical Analysis, 9(10-12): 1121-1123, 1991.
Hudson, “Infrared Systems Engineering”, Wiley-Interscience, 1969.
Jolley, et al., “Particle Concentration Fluorescence Immunoassay (PCFIA): A New, Rapid Immunoassay Technique with High Sensitivity”, 21.
Liu, et al., “Flow Injection Solid-Phase Chemiluminescent Immunoassay Using a Membrane-Based Reactor”,Analytical Chem., 63(7): 656-659, 1991.
O'Shannessy, et al., Determination of Rate and Equilibrium Binding Constants for Macromolecular Interactions Using Surface Plasmon Resonance: Use of Nonlinear Least Squares Analysis Methods,Anal. Biochem., 212: 457-468, 1993.
Perseptive Biosystems, “ID™ Real-Time Immunoassay”, Product Report, and Prec. Jul. 1, 1992.
Plant, et al., “Liposome Enhanced Flow Injection Immunoanalysis”,Biotechnology, 6: 1988.
Pollema, et al., “Flow Injection Renewable Surface Immunoassay: A New Approach to Immunoanalysis with Fluorescence Detection”,Anal. Chem., 1825-1831, 1994.
Pollema, et al., “Sequential Injection Immunoassay Utilizing Immunomagnetic Beads”,Anal. Chem.64: 1356-1361, 1992.
Rocks, et al., “Flow-Injection Analysis: A New Approach to Quantitative Measurements in Clinical Chemistry”,Clinical Chemistry, 28(3): 409-421, 1982.
Roda, et al., “Flow Systems Utilizing Nylon-Immobilized Enzymes”,Methods in Enzymology, 133: 238-248, 1986.
Rule, et al., “Flow-Injection Analysis with Chemiluminescence Detection”,Clinical Chemistry, 25(9): 1635-1638, 1979.
Sambucetti, et al., “Process for Purification of Magnetic Ink”,IBM Technical Disclose Bulletin, 18(2): 593-595, Jul. 1975.
Sato et al., “A Novel Method for Isolating Specific Endocytic Vesicles Using Very Fine Ferrite Particles Coated with Biological Ligands and the High-Gradient Magnetic Separation Technique”,J. Biochem., 100: 1481, 1986.
Shellum, et al., “Flow-Injection Immunoassays with Acridinium Ester-Based Chemiluminescence Detection”,Analytica Chimica Acta, 227: 97-107, 1989.
Skeggs, et al., “Persistence . . . and Prayer: From the Artificial Kidney to the AutoAnalyzer”,Clinical Chemistry, 46(9): 1425-1436, 2000.
Skubitz, et al., “Determination of Antibody-Hapten Association Kinetics: A Simplified Experimental Approach”,Jour. of Immunology, 114(4): 1975.
Vellom, et al., “Continous-Flow Bioluminescent Assays Employing Sepharose-Immobilized Enzymes”,Methods of Enzymolo
Glass Thomas R.
Lackie Steve J.
Choate Hall & Stewart LLP
Nguyen Bao-Thuy L.
Sapidyne Instruments Inc.
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