Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-04-15
1992-12-08
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 79, 435 792, 435 793, 435 5, 435 15, 436518, 436528, 436529, 436534, 436810, 436815, 436825, 436531, G01N 3353, G01N 33543, G01N 33573
Patent
active
051697564
ABSTRACT:
A method for the quantification or qualification of antigens with an immunological solid phase method, for example of the ELISA type, during which the invention seeks to overcome the difficulties with falsely high or falsely positive measurement results. This purpose is achieved by exposing two aliquots, preferably of the same size, of the sample containing the antigen that is to be determined to two solid phases with identical anti-antigen-antibody-coated surfaces, in which one exposure (exposure 1) occurs in the presence of antibodies present in the aliquot with the same specificity as the antibodies with which the solid phases are coated, and the other exposure (exposure 2) preferably occurs in the presence of antibodies present in the aliquot with nonspecificity for the antigen in question, but otherwise essentially the same as the antibodies present in the aliquot in exposure 1, and the antigen is quantified by means of the difference between the bonded antigen in exposure 1 and the bonded antigen in exposure 2. The method is also applicable for detecting antibodies.
REFERENCES:
patent: 4569919 (1986-01-01), Toth et al.
patent: 4661445 (1987-04-01), Saxinger et al.
Boscato, L. M. et al., Clin. Chem., 32 (8), 1491-1495 (1986, Jul.).
Clark, P. M. et al., Clin. Chem., 31 (10), 1762 (letter) 1985.
Howanitz, P. J. et al., Clin. Chem., 28 (3), 427-431 (1982).
Rijken, D. et al., Journ. Lab Clin. Med., 101, No. 2: 274-284 (1983).
Holvoet, P. et al., Biol. Abstr. 81 (8): Abstract No. 69315, (1986).
Malvano, R., "Immunoenzymatic Assay Techniques", Martinus Nijhoff publishers, The Hague/Boston/London, pp. 104-115, 1980.
Maggio, E., "Enzyme-Immunoassay", CRC Press, Inc., Boca Raton, Florida, pp. 175-176, 1980.
Bergsdorf Nils A.
Ranby Gustaf M.
Biopool International, Inc.
Kepplinger Esther L.
Scheiner Toni R.
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