Solid carriers modified with 2,4,6-trichloro-s triazine to immob

Details Solid carriers modified with 2,4,6-trichloro-s triazine to immob Solid carriers modified with 2,4,6-trichloro-s triazine to immob

1991-02-25

1993-11-30

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435179, 435180, 435181, 436529, 436530, 436531, 436532, 5253276, 530813, 530814, 530815, 530816, C12N 1110, C12N 1108, G01N 33544, G01N 33545, G07K 1700

Patent

active

052664716

ABSTRACT:
A modified solid carrier is used to covalently immobilize biomolecules such as proteins. The carrier is based on well-known matrix materials modified to have covalently bound functional groups of formula I ##STR1## that are suitable for covalent immobilization, where A is a spacer group; X is O, S, or NH and n is 0 or 1. The modified solid carrier is prepared by reacting ammonia with glycidyl groups of a carrier to form .alpha.-hydroxy-.beta.-amino groups, reacting these groups with 2,4,6-trichloro-s-triazine to form an N-triazinyl group-containing carrier, reacting this carrier with ammonia and reacting the resultant carrier with 2,4,6-trichloro-s-triazine.

REFERENCES:
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patent: 3824150 (1974-07-01), Lilly et al.
patent: 4179337 (1979-12-01), Davis et al.
patent: 4190713 (1980-02-01), Kraemer et al.
patent: 4229537 (1980-10-01), Hodgins et al.
patent: 4357311 (1982-11-01), Schutt
patent: 4511694 (1985-04-01), Kramer et al.
Nature, vol. 216, Nov. 4, 1967, pp. 514-515, G. Kay, et al. "Coupling of Enzymes to Cellulose Using Chloro-S-Triazines".

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