Sol capture immunoassay kit and procedure

Details Sol capture immunoassay kit and procedure Sol capture immunoassay kit and procedure

1992-12-30

2000-07-04

Warden, Jill

Chemistry: analytical and immunological testing

Including sample preparation

Liberation or purification of sample or separation of...

436177, 422101, B01D 3702

Patent

active

060837608

ABSTRACT:
An immunoassay procedure for detection of analytes in urine wherein an immunological reaction is conducted in an aqueous phase containing the urine and a filterable immunocomposite containing an inherently colored gold sol particle is formed if the assay is positive. The colored, gold sol particle containing immunocomposite is collected for direct visual observation on a filter element. False positive readings are minimized by (1) removing contaminants from the urine to prevent clogging of the filter element and non-specific binding of unreacted reactants thereto by contacting the urine with a porous contact element having a detergent material dispersed on its external and interstitial surfaces; (2) preventing non-specific binding of unreacted immunoreactive substances to the filter element by blocking the latter with polyvinyl pyrrolidone; and/or (3) including a green dye material in the aqueous reaction phase to improve the optical distinction after washing the collection filter between negative and positive results on the filter.

REFERENCES:
patent: 3873682 (1975-03-01), Ogawa
patent: 4357310 (1982-11-01), Chan et al.
patent: 4675299 (1987-06-01), Witty et al.
patent: 4704255 (1987-11-01), Jolley

Affiliated with

Also associated with

Rating

Sol capture immunoassay kit and procedure does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sol capture immunoassay kit and procedure, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sol capture immunoassay kit and procedure will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1485376