Sodium/proton antiporter gene

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers resistance to heat or cold

Reexamination Certificate

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C435S320100, C435S419000, C435S252300, C536S023600, C536S024300, C800S298000, C800S320000, C800S320200

Reexamination Certificate

active

06861574

ABSTRACT:
The present inventors successfully cloned the rice Na+/H+antiporter gene. It is possible to produce salt tolerant plants by using the isolated gene, or genes with equivalent functions.

REFERENCES:
patent: WO 9947679 (1999-09-01), None
Rhoads et al. Regulation of the cyanide-resistant alternative oxidase of plant mitochondria, 1998, The Journal of Biological Chemistry, vol. 273, No. 46, pp. 30750-30756.*
Hiei et al. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundarie of the T-DNA. 1994, vol. 6, No. 2, pp. 271-282.*
Sasaki et al. , Apr. 4, 2002, GenBank Accession C91832.*
Apse et al. “Cloning and Characterization of Plant Sodium/Proton Antiports”11thInternational Workshop on Plant Membrane BiologyAug. 9-14th, 1998, pp. 84.
Counillon et al. “A Point Mutation of the Na+/H+ Exchanger Gene (NHE1) and Amplification of the Mutated Allele confer Amiloride Resistance upon Chronic Acidosis”Biochemistry, May 1993, pp. 4508-4512, vol. 90, Proc. Natl. Acad. Sci., USA.
Darley et al. ANA1 a Na+/H+ Antiporter From Arabidopsis?11thInternational Workshop on Plant Membrane Biology, Aug. 9-14th1998, pp. 89.
Gaxiola et al. “TheArabidopsis ThalianaProton Transporters, AtNhx1 and Avpl, Can Function in Cation Detoxification in Yeast”Genetics, Feb. 1999, pp. 1480-1485, vol. 96, Proc. Natl. Acad. , USA.
Fukuda et al. “Molecular Cloning and Expression of the Na+/H+ Exchanger Gene inOryza Sativa” Biochimica et Biophysica Acta, 1999, pp. 149-155, Elsevier Science B.V...
Fukuda et al. “Na+/H+ Antiporter in Tonoplast Vesicles from Rice Roots”Plant Cell Physiol, 1998, vol. 39 No. 2, JSPP.
Richard Nass and Rajini Rao, “Novel Localization of a Na+/H+Exchanger in a late Endosomal Compartment of Yeast” The Journal of Biological Chemistry, Aug. 14, 1998, pp. 21054-21060, vol. 273 No. 33, The Amer. Soc. For Biochem. And Molecular Bio., Inc., USA.
Nass et al. “Intracellular Sequestration of Sodium by a Novel Na+/H+ Exchanger in Yeast Is Enhanced by Mutations in the Plasma Membrane H+ -ATPase”The Jour. Of Biological Chem., Oct. 17, 1997, pp. 26145-26152, vol. 272. No. 42, The Amer. Soc. For Biochem. And Molecular Bio., Inc., USA.
Numata et al., “Identification of A Mitochondrial Na+/H+ Exchanger”The Jour. Of Biological Chem., Mar. 20, 1998, pp. 6951-6959, vol. 273 No. 12, The Amer. Soc. For Biochem. And Molecular Bio., Inc., USA.
John Orlowski and Sergio Grinstein, “Minireview: Na+/H+ Exchangers of Mammalian Cells”The Jour. Of Biological Chem., Sep. 5, 1997, pp. 22373-22376, vol. 272 No. 36, The Amer. Soc. For Biochem. And Molecular Bio., Inc., USA.
Sasaki T et al. “Rice cDNA from Panicle” Apr. 20, 1998, Genbank Accession No. C91832.
Database EMBL 'Online! EBI; (Aug. 12, 1999) “Oryza sativa (Japonica cultivar-group) OsNHX1 mRNA,” retrieved from EMBL Database accession No. AB021878, XP002272767.
Fukuda, A. et al. “The functional analysis of the rice Na+/H+ antiporter gene,”Plant and Cell Physiology(2000), vol. 42, Supplement, p. s210: Symposia of Workshops of the 2001 Annual Meeting of the Japanese Society of Plant Physiologists; Fukuoka, Japan, Mar. 23-26, 2001.
Raush et al. “Salt stress responses of higher plants: the role of proton pumps and Na+/H+-antiporters,”Journal of Plant Physiology(1996), vol. 148, No. 3/4, pp. 425-433.

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