Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1994-09-09
1996-10-08
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
435 691, 4352523, 43525231, 4353201, 536 221, 536 234, 536 237, C07K 100, C12N 120, C07H 1900, C07H 2100
Patent
active
055632467
ABSTRACT:
The present invention relates to the cloning and expression of a sodium ion binding protein. In particular, the invention relates to cloning and expression of a nhaS gene. The nhaS gene product, NhaS, is a protein characterized by binding to and sequestering sodium ion (Na.sup.+). The invention further relates to functional fragments of a sodium ion binding protein, which fragments are characterized by their ability to bind to sodium ion. In a specific embodiment, the fragment is a fragment of NhaS. The gene encoding the sodium binding protein can be introduced into cells to produce desalination bioreactors. The gene encoding the sodium binding protein can also be introduced into plants as a transgene to produce plants that are resistant to sodium. The sodium binding protein itself may be used for treatments involving Na.sup.+ /K.sup.+ ATPase disorders, e.g., in heart disease; the protein also may be introduced parenterally, preferably orally, to bind to and sequester dietary sodium.
REFERENCES:
Glover, 1984, "Principle of Cloning DNA" in Gene Cloning: The Mechanics of DNA Manipulations, Chapman and Hall Ltd., New York, pp. 1-20.
Green et al., 1986, "The Role of Antisense RNA in Gene Regulation" Ann. Rev. Biochem. 55:569-97.
Glover, 1984, "Expression of Cloned DNAs in E. coli plasmids" in Gene Cloning: The Mechanics of DNA Manipulations, Chapman and Hall Ltd., New York, pp. 110-127.
Aronson, P. S., 1985 "Kinetic Properties of the Plasma Membrane Na.sup.+ -H.sup.+ Exchange" Ann. Rev. Physiol. 47:545-560.
Shull, G. E. et al., 1986, "Molecular Cloning of Three Distinct Forms of the Na.sup.+, K.sup.+ -ATPase .alpha.-Subunit From Rat Brain" Biochemistry 25:8125-8132.
Krulwich, T. A. and Guffanti, A. A. 1989, "Alkalophillic Bacteria" Annu. Rev. microbiol. 43:435-63.
Sardet, C. et al., 1989, "Molecular Cloning, Primary Structure, and Expression of the Human Growth Factor-Activatable Na.sup.+ /H.sup.+ Antiporter" Cell 56:271-280.
Padan, E. et al., 1989, "Deletion of ant in Escherichia coli Reveals its Function in Adaptation to High Salinity and an Alternative Na.sup.+ /H.sup.+ Antiporter System" J. Biological Chemistry 264:20297-20302.
Nicoll, D. A. et al., 1990, "Molecular Cloning and Functional Expression of the Cardiac Sarcolemmal Na.sup.+ -Ca.sup.+ Exchanger" Science 250:562-565.
Ivey, D. M. et al., 1991, "Molecular Cloning and Sequencing of a Gene from Alkalphillic Bacillus firmus OF4 That Functionally Complements and Escherichia coli Strain Carrying a Deletion in the nhaA Na.sup.' /H.sup.' Antiporter Gene" J. Biological Chemistry 266:23483-23489.
Ivey D. Mack
Krulwich Terry A.
Kim Hyosuk
Mount Sinai School Of Medicine Of The City of New York
Wax Robert A.
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