Small triplex forming PNA oligos

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091100, C536S023100

Reexamination Certificate

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06979536

ABSTRACT:
A method for the determination of nucleic acids which is highly specific and simple comprises the formation of a triple stranded binding complex including two separate, different probe molecules and detecting the formation of the complex via the inclusion of one of the probes. The method can be used to differentiate between nucleic acids having a single base difference in sequence.

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patent: WO 92/20702 (1992-11-01), None
Corey, David, “Peptide nucleic acids: expanding the scope of nucleic acid recognition”, Trends Biotechnology, 15 (6): 224-229, Jun. 1997.
Svinarchuk, Fedor, “An Unusually Stable Purine(Purine-Pyrimidine) Short Triplex”, J. Biol Chem, 270(23): 14068-71, Jun. 9, 1995.
International Publication No. WO 96/02558 published Feb. 1, 1996.
International Publication No. WO 95/01370 published Jan. 12, 1995.

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